Comparison of edwardsiella ictaluri isolates from different hosts and. This thesis does not include proprietary or classified information. Edwardsiellosis caused by edwardsiella ictaluri in laboratory. Edwardsiellosis caused by edwardsiella ictaluri in laboratory populations of zebrafish danio rerio article pdf available in journal of aquatic animal health 253. As soon as the fish shows interest and attacks, let go of the food. Edwardsiellosis caused by edwardsiella ictaluri in.
Pathogenesis of and strategies for preventing edwardsiella. The type species is edwardsiella tarda, which is occasionally isolated from the stools of both healthy humans and those with diarrhea, from the blood of humans and other animals, and from human urine. Wild hosts include european and japanese eels, largemouth bass, striped sea bass, atlantic salmon, marble goby, snakes and birds. Klesius aquatic animal health research unit, usdaars, msa, 990 wire road, auburn, alabama 368310952 usa abstract. The genus edwardsiella includes two species of bacteria that cause major diseases in fish. Zebra nerite snails are the most popular species of freshwater nerite snail in the aquarium hobby. Pathology associated with edwardsiella ictaluri in catfish ietalurus punctatus and danio danio devario. Sixtyeight gramnegative bacteria were isolated from cultures from these 52 water samples, of which 48 were identified as enterobacteriaceae. Zebra technologies zebra bar code printing solution validated. The bacterium was not found in fishes sampled from any of the other 14 sites. A genus of gramnegative, facultatively anaerobic bacteria family enterobacteriaceae containing motile, peritrichous, nonencapsulated rods. One of the fish in the same group died 3 days after the inoculation and the other 2 died on the fourth day.
Infection causes edwardsiella septicemia also known as es, edwardsiellosis, emphysematous putrefactive disease of catfish, fish gangrene, and red disease in channel catfish, eels, and flounder. Edwardsiella ictaluri also known as enteric septicaemia of catfish, hole in the head disease, and esc is a member of the family hafniaceae. Here we developed a protocol to raise microbefree zebrafish larvae fed on. Edwardsiella ictaluri bacteraemia elicits shedding of aeromonas hydrophila complex in latently infected channel catfish, ictalurus punctatus rafinesque. Edwardsiella tarda is one of the serious fish pathogens, infecting both cultured and wild fish species.
Edwardsiellosis caused by edwardsiella ictaluri in laboratory populations of zebrafish danio rerio. However in large numbers they will require extra food. The genus edwardsiella includes two species of bacteria that cause major disease in fish. Edwardsiella ictaluri scientific classification domain. Survival of bacterial and parasitic pathogens from zebrafish danio. Experimental edwardsiella ictaluri infection causes mortality in white perch morone americana edwardsiella ictaluri, the causative agent of enteric septicemia of catfish esc has been isolated from other fish species from ten families, but not white perch morone americana. First detection of edwardsiella ictaluri proteobacteria.
A guide for the laboratory use of zebrafish danio brachydanio rerio. Edwardsiella tarda is predominantly found in freshwater environments colonizing the guts of fish, including flounder living off the coasts of east asia and catfish that live in regions of the united states. Edwardsiella ictaluri belongs to the enterobacteriaceae family and is a gram negative, short, pleomorphic rod, measuring 0. The zebra moray is a benthic fish, its favorite habitat corresponds to the rocky or coral reef on coastal shallow water between 1 and 39 meters deep. It causes the disease enteric septicaemia of catfish, which infects a variety of fish species.
A third species, edwardsiella hoshinae grimont et al. Zebrafish are generally quite resistant to opportunistic gram negative bacteria, such as aeromonas and pseudomonas species, but e. We evaluated mycobacterium chelonae, mycobacterium marinum, and edwardsiella ictaluri, each originally isolated from zebrafish, eggs of. Oct 04, 2012 edwardsiella tarda is one of the serious fish pathogens, infecting both cultured and wild fish species.
Growth response and resistance to edwardsiella ictaluri of channel cat. Zebra fish research list of high impact articles ppts. Edwardsiella ictaluri an overview sciencedirect topics. The bacterium is a facultatively anaerobic, small, motile, gram negative, straight rod with flagella.
Edwardsiella article about edwardsiella by the free dictionary. Natural transmission occurs primarily through shedding of bacteria from carriers or diseased fish, or by cannibalism of dead fish. Except where reference is made to the work of others, the work described in this thesis is my own or was done in collaboration with my advisory committee. Although esc may occur in healthy fish in nonstressful environmental conditions, stress factors such as handling, close. The fish in groups ii and iii did not develop any clinical signs of the disease and remained alive. Isolation and characterization of edwardsiella ictaluri from. Initially, this pathogen was considered to be hostspecific for catfish species, but has recently been found in other noncatfish species, including research zebrafish noga, 2010.
The zebrafish book a guide for the laboratory use of. The zebra moray is widespread throughout the indopacific area from eastern coast of africa until western coast of the americas, including the red sea, hawaii and galapagos. Cause of esc enteric septicemia of catfish can occur when a susceptible host channel catfish encounters a virulent pathogen edwardsiella ictaluri under environmental conditions that are conducive to proliferation of the pathogen and stressful for the host. Edwardsiella ictaluri, a gramnegative rod, is the etiologic agent of enteric septicemia of catfish esc hawke et al. They are gram negative, usually motile and facultatively anaerobic. It is a primary pathogen, and unlike aeromonads, etc. Isolation and characterization of edwardsiella ictaluri from cultured yellow catfish pelteobagrus fulvidraco geng yi1,2, wang kaiyu1,2, chen defang1, fan fanling1, huang yidan1 1 research center of fish disease, sichuan agricultural university, yaan, sichuan 625014, p.
Edwardsiella ictaluriinfection in rainbow trout oncorhynchus. The use of signaturetagged mutagenesis in a waterborne infection model resulted in the identification of 50 mutants that were unable to infectsurvive in catfish. Its pathology is such that it causes chronic encephalitis or acute septicemias. Zebra nerite snails are excellent at consuming algae and organics. The most relevant species of the genus edwardsiella is edwardsiella tarda ewing et al 1965. Edwardsiella ictaluri is a bacterium that causes enteric septicaemia of catfish esc disease. Edwardsiella ictaluri from walking catfish, clarias batrachus l. As zebra fish is also vertebrate like humans their studies will be closely related to humans. This species is associated with fresh water ecosystems.
In aquaculture, its hosts include white, channel, walking and sutchi catfish species. Api if isolates are tested with the col systemmm api described in ercia section 2, 3. Zebra nerite snail neritina zebra 1 fish 2 fish dartmouth. We report the first cases of edwardsiella ictaluri causing epizootics in laboratory populations of zebrafish danio rerio. Edwardsiella tarda is a member of the family hafniaceae. Hawke jp1, kent m, rogge m, baumgartner w, wiles j. Zebra lionfish dendrochirus zebra in aquarium reef app.
First report of edwardsiella ictaluri in a species other than channel catfish. The bacterium is a short, gram negative, pleomorphic rod with flagella. Although recent progress in edwardsiellosis research has enabled the development of. Quiniou usdaars theresa cody florida fish and wildlife conservation commission. Edwardsiella ictaluri was consistently isolated from the spleens, livers, and head kidneys of diseased nile tilapia oreochromis niloticus from a farm experiencing mortality events in several. Pdf edwardsiella ictaluri as the causative agent of. Edwardsiella ictaluri from walking catfish, clarias batrachus. Progress 090102 to 083106 outputs edwardsiella ictaluri is the leading cause of mortality in channel catfish culture, but little is known about its pathogenesis.
Edwardsiella ictaluri is primarily recognized as a disease of catfish species and is known to cause an economically important bacterial disease of farmraised catfish in the usa and abroad. They will try to eat all fish, shrimps and similar life forms that are small enough to fit in itheir mouth. Edwardsiella ictaluri isolates from zebrafish are believed to comprise a unique group and were differentiated from catfish isolates by exhibiting weaker motility, autoaggregation in broth, a different plasmid profile two plasmids of 4. Growth response and resistance to edwardsiella ictaluri. Enteric septicemia of catfish can occur when a susceptible host channel catfish encounters a virulent pathogen edwardsiella ictaluri under the right environmental conditions. Identification and characterization of edwardsiella ictaluri. A2 profiles obtained with api20e for known fish pathogens appendix 2.
They are easy to maintain, manipulate, and observe in the lab. The extracellular secretion of super oxide anion and hydrogen peroxide was increased in vaccinated fish from the combo and commer cial groups but not in the fish fed beef tallow. Identification of edwardsiella ictaluri virulence factors. Early detection of edwardsiella ictaluri continued from page 1 florfenicol antibiotic studies conducted pat gaunt water temperatures dropped below the ideal temperature range for e. The fish and shrimps used as foods, must remain whole to give the lionfish the optimum nutrition. Pdf edwardsiellosis caused by edwardsiella ictaluri in. In 1976 there were observed natural outbreaks of e. Browse the independents complete collection of articles and commentary on zebra fish. Experimental edwardsiella ictaluri infection causes. Comparative analysis of edwardsiella isolates from fish in the eastern united states identifies two distinct genetic taxa amongst organisms phenotypically classified as e. They can be identified by their distinct black and gold stripes running from a point at its shells. Edwardsiella septicaemia edwardsiella tarda infection. Abstract we report the first cases of edwardsiella ictaluri causing epizootics in laboratory populations of zebrafish danio rerio.
Edwardsiella ictaluri definition of edwardsiella ictaluri. Edwardsiella ictaluri is a gramnegative bacterium which causes enteric septicemia in majority of catfish species and japanese eels anguilla japonica in japan and taiwan and infecting fish only hawke et al. It can also be found in the intestinal tract of birds, reptiles, and mammals janda et. Our bar code, card, kiosk, and rfid printers, as well as realtime location solutions, have manage critical assets, people, and transactions for improved business efficiencies. Breeding and getting eggs from the zebra fish is relatively easy. Although esc may occur in healthy fish in nonstressful environmental con. Edwardsiella tarda is a gramnegative intracellular pathogen accounting for enormous economic losses in cultured seawater and freshwater fish. Edwardsiella definition of edwardsiella by medical dictionary.
712 625 1410 783 344 941 555 481 636 1509 1159 1429 165 816 1118 783 317 384 977 1243 452 67 1107 955 124 1227 1320 620 1146 1175 138 955 196 596 90 762 948 1336 1419 952 1182